Job Opportunities
Research Associate
Area(s) of expertise desired: Biochemistry, Molecular Biology, Protein Chemistry
Description
ProtElix, Inc., an early stage, pre-IPO startup, is a proven pioneer in the field of protein engineering and optimization by molecular evolution. We're building upon our extensive, patented technology and the latest advances in evolutionary mutagenesis to address critical unmet needs. Current projects are focused on developing optimized enzymes, and next generation protein-based therapeutics.
Our biofuel and clinical enzyme research team is growing, and we are currently seeking a Research Associate with a strong background in molecular biology and protein characterization. Primarily, this position will involve building protein libraries through ProtElix' proprietary mutagenesis technology to improve an enzyme’s thermostability profile.
Requirements
This position requires three to five years of industry laboratory experience, as well as a BS or MS degree.
Must have knowledge in molecular biology and experience constructing vectors for various expression systems (S. cerevisiae, P. Pastoris, E. Coli). Previous experience building libraries is a plus. Experience with high throughput functional screening of protein libraries is preferred. Candidate should also have significant biochemical experience working with proteins and have a strong knowledge of basic protein-chemistry skills including ELISA, Western blots and quantitation techniques. Also, experience with RT-PCR (Taqman) and liquid handling robotics is desirable.
You must be detail-oriented and diligent in performing experimental procedures and keeping current documentation in laboratory notebooks. Technical proficiency and ability to troubleshoot experiments is required. Good sterile laboratory techniques, media preparation and lab organizational skills, including ordering supplies from vendors, are essential. Proficiency in molecular biology software for sequence analysis (Sequencher, MacVector), writing SOPs, and the ability to work well in a team environment are essential.
This is a full-time position offering competitive salary and top-notch benefits, including: medical, dental, life, and long-term disability insurance; a 401(k) retirement plan; an incentive stock option program; flextime; ten paid holidays; three weeks of PTO; and medical & dependent care flexible spending accounts.
To apply for this position, please send your CV/resume and a cover letter to hr@protelix.com.
ProtElix is an equal opportunity employer.
Staff Scientist
Job duties – Conduct independent research contributing to identification and development of novel therapeutics. Contribute to the company's overall research strategy. Apply protein structure-based perspective to design of protein scaffold and libraries. Utilize mutagenesis libraries and high-throughput assays to screen for optimized protein characteristics. Design, optimize and execute protein purification strategies to support in vitro and in vivo studies. Utilize standard molecular biology techniques to clone and manipulate DNA (DNA and protein analysis software, PCR design and optimization, cloning strategies). Plan experiments, conduct trouble-shooting and problem solving, communicate research strategy and results. Conduct assays of thermal stability of recombinant proteins. Perform real-time kinetic analysis of protein-protein interactions. Develop ELISA-based and cell-based assays for competitive inhibition of cytokine activity.
Education - Ph.D. degree or foreign equivalent in Biochemistry, Molecular Biology, Immunology, or related Biological Science.
Experience - 5 years experience in Post-doctoral research in Biochemistry, Molecular Biology, Immunology, or related biological field, to include a minimum of two years in a biotechnology industry setting.
Additional requirements:
Familiarity in DNA manipulation (including cloning strategies and (PCR) Polymerase Chain Reaction);
In-depth knowledge of protein expression in bacterial and eukaryotic systems;
Expertise in establishing and optimizing protein purification protocols;
Familiarity with protein structure viewing and analysis software and databases;
Background conducting studies on protein thermal stability;
Theoretical understanding and hands-on background with real-time analysis of kinetics of protein-protein interactions;
Proven ability to design and execute cell-based assays of cytokine function;
Ability to work independently, directing and conducting research projects, involving experimental design and data analysis; and
Demonstrated skill in written and oral scientific communication.
Location – Hayward, CA
How to apply – By mail to Job #PK, att’n: Jeff Mace, Protelix, Inc., 26118 Research Road, Hayward, CA 94545.
